2np0

X-ray diffraction
2.62Å resolution

Crystal structure of the Botulinum neurotoxin type B complexed with synaptotagamin-II ectodomain

Released:

Function and Biology Details

Reaction catalysed:
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates.
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Synaptotagmin-2 Chain: B
Molecule details ›
Chain: B
Length: 21 amino acids
Theoretical weight: 2.54 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P46097 (Residues: 40-60; Coverage: 5%)
Gene name: Syt2

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: C2221
Unit cell:
a: 170.415Å b: 351.971Å c: 101.968Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.227
Expression system: Not provided