Solution NMR

1H, 13C and 15N chemical shift assignments and solution structure for PARP-1 F1F2 domains in complex with a DNA single-strand break


Function and Biology Details

Reaction catalysed:
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Poly [ADP-ribose] polymerase 1 Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 24.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P09874 (Residues: 1-214; Coverage: 21%)
Gene names: ADPRT, PARP1, PPOL
Sequence domains: Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
Structure domains: Zinc finger, PARP-type
DNA (45-MER) Chain: B
Molecule details ›
Chain: B
Length: 45 nucleotides
Theoretical weight: 13.87 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 29%
Refinement method: simulated annealing
Chemical shifts: BMR25888  
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided