2n7l

Solution NMR

NMR structure of the N-domain of troponin C bound to the switch region of troponin I and the covalent levosimendan analog i9

Released:
Source organism: Homo sapiens
Entry authors: Pineda Sanabria SE, Sykes BD, Robertson IM

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Troponin C, slow skeletal and cardiac muscles; Troponin I, cardiac muscle Chain: C
Molecule details ›
Chain: C
Length: 141 amino acids
Theoretical weight: 16.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19429 (Residues: 145-174; Coverage: 14%)
  • Canonical: P63316 (Residues: 1-89; Coverage: 55%)
Gene names: TNNC, TNNC1, TNNI3
Sequence domains: EF-hand domain pair

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 71%
Refinement method: simulated annealing
Chemical shifts: BMR25810  
Expression system: Escherichia coli