› 2mze

Solution NMR

NMR Solution Structure of the PRO Form of Human Matrilysin (proMMP-7)

Released:
DOI: 10.2210/pdb2mze/pdb
PDB entry 2mze coloured by chain, ensemble of 20 models, front view.
PDB entry 2mze coloured by chain, ensemble of 20 models, side view.
PDB entry 2mze coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Charge-Triggered Membrane Insertion of Matrix Metalloproteinase-7, Supporter of Innate Immunity and Tumors.
Prior SH, Fulcher YG, Koppisetti RK, Jurkevich A, Van Doren SR
Structure 23 2099-110 (2015)
PMID: 26439767

Function and Biology Details

Reaction catalysed: 
Cleavage of 14-Ala-|-Leu-15 and 16-Tyr-|-Leu-17 in B chain of insulin. No action on collagen types I, II, IV, V. Cleaves gelatin chain alpha-2(I) > alpha-1(I).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Matrilysin Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 27.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09237 (Residues: 18-267; Coverage: 100%)
Gene names: MMP7, MPSL1, PUMP1
Sequence domains:
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 84%
Refinement method: simulated annealing, torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Therapeutic Potential of Matrix Metalloproteinase Inhibition in Breast Cancer.
Radisky et al. (2017)
3 more

5 mentions without citation

Role of Matrix Metalloproteinases in Angiogenesis and Cancer.
Quintero-Fabián et al. (2019)
4 more