2mpt

Solution NMR

WW3 domain of Nedd4L in complex with its HECT domain PY motif

Released:

Function and Biology Details

Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase NEDD4-like Chain: A
Molecule details ›
Chain: A
Length: 48 amino acids
Theoretical weight: 5.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96PU5 (Residues: 496-539; Coverage: 5%)
Gene names: KIAA0439, NEDD4L, NEDL3
Sequence domains: WW domain
E3 ubiquitin-protein ligase NEDD4-like Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.61 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q96PU5 (Residues: 945-957; Coverage: 1%)
Gene names: KIAA0439, NEDD4L, NEDL3

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 53%
Refinement method: DGSA-distance geometry simulated annealing
Chemical shifts: BMR25000  
Expression systems:
  • Escherichia coli
  • Not provided