2mf0

Solution NMR

Structural basis of the non-coding RNA RsmZ acting as protein sponge: Conformer L of RsmZ(1-72)/RsmE(dimer) 1to3 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Translational regulator CsrA1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 70 amino acids
Theoretical weight: 7.85 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DPC3 (Residues: 1-59; Coverage: 92%)
Gene names: csrA1, rsmE
Sequence domains: Global regulator protein family
RNA_(72-MER) Chain: G
Molecule details ›
Chain: G
Length: 72 nucleotides
Theoretical weight: 23.37 KDa
Source organism: Pseudomonas fluorescens
Expression system: Not provided
Sequence domains: PrrB/RsmZ RNA family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 6%
Refinement method: simulated annealing
Chemical shifts: BMR19534  
Expression systems:
  • Escherichia coli
  • Not provided