2mak

Solution NMR

Solution structure of the STIM1 CC1-CC2 homodimer in complex with two Orai1 C-terminal domains.

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Stromal interaction molecule 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 82 amino acids
Theoretical weight: 9.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13586 (Residues: 312-387; Coverage: 12%)
Gene names: GOK, STIM1
Calcium release-activated calcium channel protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 23 amino acids
Theoretical weight: 2.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96D31 (Residues: 272-292; Coverage: 7%)
Gene names: CRACM1, ORAI1, TMEM142A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 42%
Refinement method: simulated annealing
Chemical shifts: BMR19363  
Expression system: Escherichia coli