› 2mak

Solution NMR

Solution structure of the STIM1 CC1-CC2 homodimer in complex with two Orai1 C-terminal domains.

Released:
DOI: 10.2210/pdb2mak/pdb
PDB 2mak coloured by chain and viewed from the front.
PDB 2mak coloured by chain and viewed from the side.
PDB 2mak coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
STIM1/Orai1 coiled-coil interplay in the regulation of store-operated calcium entry.
Stathopulos PB, Schindl R, Fahrner M, Zheng L, Gasmi-Seabrook GM, Muik M, Romanin C, Ikura M
OpenAccess logo Nat Commun 4 2963 (2013)
PMID: 24351972

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Stromal interaction molecule 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 82 amino acids
Theoretical weight: 9.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13586 (Residues: 312-387; Coverage: 12%)
Gene names: GOK, STIM1
Calcium release-activated calcium channel protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 23 amino acids
Theoretical weight: 2.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96D31 (Residues: 272-292; Coverage: 7%)
Gene names: CRACM1, ORAI1, TMEM142A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 42%
Refinement method: simulated annealing
Chemical shifts: BMR19363  
Expression system: Escherichia coli

Quick links

Citations

33 review citations

The Orai Pore Opening Mechanism.
Tiffner et al. (2021)
32 more

4 mentions without citation

Store-Operated Calcium Channels.
Prakriya et al. (2015)
3 more