› 2m00

Solution NMR

Solution structure of staphylococcal nuclease E43S mutant in the presence of ssDNA and Cd2+

Released:
DOI: 10.2210/pdb2m00/pdb
PDB entry 2m00 coloured by chain, ensemble of 20 models, front view.
PDB entry 2m00 coloured by chain, ensemble of 20 models, side view.
PDB entry 2m00 coloured by chain, ensemble of 20 models, top view.
Source organism: Staphylococcus aureus subsp. aureus MW2
Primary publication:
Modeling of the [E43S]SNase-ssDNA-Cd(2+) complex: structural insight into the action of nuclease on ssDNA.
Xie T, Feng Y, Shan L, Wang J
Arch Biochem Biophys 532 103-13 (2013)
PMID: 23416741

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermonuclease Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.78 KDa
Source organism: Staphylococcus aureus subsp. aureus MW2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NXI6 (Residues: 80-228; Coverage: 73%)
Gene names: MW0769, nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 86%
Refinement method: molecular dynamics, simulated annealing
Expression system: Escherichia coli

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