Solution NMR

Structure of the gp78 CUE domain


Function and Biology Details

Reaction catalysed:
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
E3 ubiquitin-protein ligase AMFR Chain: C
Molecule details ›
Chain: C
Length: 52 amino acids
Theoretical weight: 5.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q9UKV5 (Residues: 453-504; Coverage: 8%)
Gene names: AMFR, RNF45
Sequence domains: CUE domain
Structure domains: DNA helicase RuvA subunit, C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 90%
Refinement method: simulated annealing
Chemical shifts: BMR18581  
Expression system: Escherichia coli