› 2lup

Solution NMR

RDC refined solution structure of double-stranded RNA binding domain of S. cerevisiae RNase III (rnt1p) in complex with the terminal RNA hairpin of snr47 precursor

Released:
DOI: 10.2210/pdb2lup/pdb
PDB entry 2lup coloured by chain, ensemble of 16 models, front view.
PDB entry 2lup coloured by chain, ensemble of 16 models, side view.
PDB entry 2lup coloured by chain, ensemble of 16 models, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structure of a yeast RNase III dsRBD complex with a noncanonical RNA substrate provides new insights into binding specificity of dsRBDs.
Wang Z, Hartman E, Roy K, Chanfreau G, Feigon J
Structure 19 999-1010 (2011)
PMID: 21742266

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169729 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease 3 Chain: B
Molecule details ›
Chain: B
Length: 90 amino acids
Theoretical weight: 9.82 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q02555 (Residues: 366-453; Coverage: 19%)
Gene names: RNT1, YM9408.01C, YM9959.21, YMR239C
Sequence domains: Double-stranded RNA binding motif
Structure domains: Double Stranded RNA Binding Domain
RNA (32-MER) Chain: A
Molecule details ›
Chain: A
Length: 32 nucleotides
Theoretical weight: 10.31 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 71%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

14 review citations

How RNA-Binding Proteins Interact with RNA: Molecules and Mechanisms.
Corley et al. (2020)
13 more

2 mentions without citation

Intrinsic dynamics of an extended hydrophobic core in the S. cerevisiae RNase III dsRBD contributes to recognition of specific RNA binding sites.
Hartman et al. (2013)
1 more