2ltq

Solid-state NMR

High resolution structure of DsbB C41S by joint calculation with solid-state NMR and X-ray data

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Disulfide bond formation protein B Chains: A, D
Molecule details ›
Chains: A, D
Length: 176 amino acids
Theoretical weight: 20.11 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6M2 (Residues: 1-176; Coverage: 100%)
Gene names: JW5182, b1185, dsbB, roxB, ycgA
Sequence domains: Disulfide bond formation protein DsbB
Structure domains: DsbB-like
Fab fragment light chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 239 amino acids
Theoretical weight: 26.36 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab fragment heavy chain Chains: C, F
Molecule details ›
Chains: C, F
Length: 221 amino acids
Theoretical weight: 23.67 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 2%
Refinement method: simulated annealing
Chemical shifts: BMR18493  
Expression system: Escherichia coli