2lrl

Solution NMR

Solution Structures of the IIA(Chitobiose)-HPr complex of the N,N'-Diacetylchitobiose Branch of the Escherichia coli Phosphotransferase System

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PTS system N,N'-diacetylchitobiose-specific EIIA component Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 103 amino acids
Theoretical weight: 11.26 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P69791 (Residues: 14-116; Coverage: 89%)
Gene names: JW1725, b1736, celC, chbA
Sequence domains: PTS system, Lactose/Cellobiose specific IIA subunit
Structure domains: Phosphotransferase system, lactose/cellobiose-type IIA subunit
Phosphocarrier protein HPr Chain: D
Molecule details ›
Chain: D
Length: 85 amino acids
Theoretical weight: 9.13 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA04 (Residues: 1-85; Coverage: 100%)
Gene names: JW2408, b2415, hpr, ptsH
Sequence domains: PTS HPr component phosphorylation site
Structure domains: HPr-like

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 37%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli