› 2lpz

Solid-state NMR

Atomic model of the Type-III Secretion System Needle

Released:
DOI: 10.2210/pdb2lpz/pdb
PDB entry 2lpz coloured by chain, ensemble of 10 models, front view.
PDB entry 2lpz coloured by chain, ensemble of 10 models, side view.
PDB entry 2lpz coloured by chain, ensemble of 10 models, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Atomic model of the type III secretion system needle.
Loquet A, Sgourakis NG, Gupta R, Giller K, Riedel D, Goosmann C, Griesinger C, Kolbe M, Baker D, Becker S, Lange A
Nature 486 276-9 (2012)
PMID: 22699623

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 29-mer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SPI-1 type 3 secretion system needle filament protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c
Length: 80 amino acids
Theoretical weight: 8.86 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41784 (Residues: 1-80; Coverage: 100%)
Gene names: STM2873, prgI, sctF1
Sequence domains: Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 2%
Refinement method: Monte Carlo and gradient-based optimization
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

51 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
50 more

18 mentions without citation

Principles for Integrative Structural Biology Studies.
Rout et al. (2019)
17 more