2lpb

Solution NMR

Structure of the complex of the central activation domain of Gcn4 bound to the mediator co-activator domain 1 of Gal11/med15

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mediator of RNA polymerase II transcription subunit 15 Chain: A
Molecule details ›
Chain: A
Length: 81 amino acids
Theoretical weight: 9.52 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P19659 (Residues: 158-238; Coverage: 8%)
Gene names: ABE1, GAL11, MED15, RAR3, SDS4, SPT13, YOL051W
Structure domains: Helix Hairpins
General control transcription factor GCN4 Chain: B
Molecule details ›
Chain: B
Length: 34 amino acids
Theoretical weight: 3.96 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P03069 (Residues: 101-134; Coverage: 12%)
Gene names: AAS101, AAS3, ARG9, GCN4, YEL009C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 87%
Refinement method: simulated annealing
Chemical shifts: BMR18244  
Expression system: Escherichia coli