› 2lpb

Solution NMR

Structure of the complex of the central activation domain of Gcn4 bound to the mediator co-activator domain 1 of Gal11/med15

Released:
DOI: 10.2210/pdb2lpb/pdb
PDB entry 2lpb coloured by chain, ensemble of 13 models, front view.
PDB entry 2lpb coloured by chain, ensemble of 13 models, side view.
PDB entry 2lpb coloured by chain, ensemble of 13 models, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
The acidic transcription activator Gcn4 binds the mediator subunit Gal11/Med15 using a simple protein interface forming a fuzzy complex.
Brzovic PS, Heikaus CC, Kisselev L, Vernon R, Herbig E, Pacheco D, Warfield L, Littlefield P, Baker D, Klevit RE, Hahn S
Mol Cell 44 942-53 (2011)
PMID: 22195967

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136526 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mediator of RNA polymerase II transcription subunit 15 Chain: A
Molecule details ›
Chain: A
Length: 81 amino acids
Theoretical weight: 9.52 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P19659 (Residues: 158-238; Coverage: 8%)
Gene names: ABE1, GAL11, MED15, RAR3, SDS4, SPT13, YOL051W
Sequence domains: Gal11 activator-binding domain (ABD1)
Structure domains: Helix Hairpins
General control transcription factor GCN4 Chain: B
Molecule details ›
Chain: B
Length: 34 amino acids
Theoretical weight: 3.96 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P03069 (Residues: 101-134; Coverage: 12%)
Gene names: AAS101, AAS3, ARG9, GCN4, YEL009C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 87%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

24 review citations

The Mediator complex and transcription regulation.
Poss et al. (2013)
23 more

12 mentions without citation

Mechanisms of Macromolecular Interactions Mediated by Protein Intrinsic Disorder.
Hong et al. (2020)
11 more