Function and Biology Details
Reaction catalysed:
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Chemotaxis phosphorelay complex CheA-CheY (preferred)
PDBe Complex ID:
PDB-CPX-139568 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chemotaxis protein CheA
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 24.99 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
Sequence domains:
Structure domains:
Length: 225 amino acids
Theoretical weight: 24.99 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
- Canonical:
P07363 (Residues: 1-225; Coverage: 34%)
Sequence domains:
Structure domains:
Chemotaxis protein CheY
Molecule details ›
Chain: Y
Length: 128 amino acids
Theoretical weight: 13.98 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold
Length: 128 amino acids
Theoretical weight: 13.98 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
- Canonical: P0AE67 (Residues: 2-129; Coverage: 99%)
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Chemical shift assignment:
5%
Refinement method:
torsion angle dynamics
Expression system: Escherichia coli
