2leg

Solid-state NMR

Membrane protein complex DsbB-DsbA structure by joint calculations with solid-state NMR and X-ray experimental data

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thiol:disulfide interchange protein DsbA Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 21.12 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEG4 (Residues: 20-208; Coverage: 100%)
Gene names: JW3832, b3860, dsbA, dsf, ppfA
Sequence domains: DSBA-like thioredoxin domain
Structure domains: Glutaredoxin
Disulfide bond formation protein B Chain: B
Molecule details ›
Chain: B
Length: 176 amino acids
Theoretical weight: 20.11 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6M2 (Residues: 1-176; Coverage: 100%)
Gene names: JW5182, b1185, dsbB, roxB, ycgA
Sequence domains: Disulfide bond formation protein DsbB
Structure domains: DsbB-like

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 36%
Refinement method: simulated annealing
Chemical shifts: BMR17710  
Expression system: Escherichia coli