› 2lcq

Solution NMR

Solution structure of the endonuclease Nob1 from P.horikoshii

Released:
DOI: 10.2210/pdb2lcq/pdb
PDB entry 2lcq coloured by chain, ensemble of 20 models, front view.
PDB entry 2lcq coloured by chain, ensemble of 20 models, side view.
PDB entry 2lcq coloured by chain, ensemble of 20 models, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Structural and functional analysis of the archaeal endonuclease Nob1.
Veith T, Martin R, Wurm JP, Weis BL, Duchardt-Ferner E, Safferthal C, Hennig R, Mirus O, Bohnsack MT, Wöhnert J, Schleiff E
Nucleic Acids Res 40 3259-74 (2012)
PMID: 22156373

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129726 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoribonuclease Nob1 Chain: A
Molecule details ›
Chain: A
Length: 165 amino acids
Theoretical weight: 18.33 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O58440 (Residues: 1-161; Coverage: 100%)
Gene names: PH0709, nob1, vapC6
Sequence domains: PIN domain of ribonuclease
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 89%
Refinement method: torsion angle dynamics, molecular dynamics
Expression system: Escherichia coli

Quick links

Citations

8 review citations

DExD/H-box RNA helicases in ribosome biogenesis.
Martin et al. (2013)
7 more

8 mentions without citation

Structural conservation of the PIN domain active site across all domains of life.
Senissar et al. (2017)
7 more