› 2lb9

Solution NMR

Refined solution structure of a cyanobacterial phytochrome gaf domain in the red light-absorbing ground state (corrected pyrrole ring planarity)

Released:
DOI: 10.2210/pdb2lb9/pdb
PDB entry 2lb9 coloured by chain, ensemble of 20 models, front view.
PDB entry 2lb9 coloured by chain, ensemble of 20 models, side view.
PDB entry 2lb9 coloured by chain, ensemble of 20 models, top view.
Source organism: Synechococcus sp. JA-2-3B'a(2-13)
Primary publication:
Solution structure of a cyanobacterial phytochrome GAF domain in the red-light-absorbing ground state.
Cornilescu G, Ulijasz AT, Cornilescu CC, Markley JL, Vierstra RD
J Mol Biol 383 403-13 (2008)
PMID: 18762196

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173663 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 23.34 KDa
Source organism: Synechococcus sp. JA-2-3B'a(2-13)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2JIZ5 (Residues: 1-200; Coverage: 24%)
Gene name: CYB_2465
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments

1 bound ligand:
Ligand CYC 1 x CYC
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 64%
Refinement method: molecular dynamics, torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structure and function of plant photoreceptors.
Möglich et al. (2010)
5 more

1 mention without citation

The D-ring, not the A-ring, rotates in Synechococcus OS-B' phytochrome.
Song et al. (2014)