Solution NMR

Solution NMR Structure of Med25(391-543) Comprising the Activator-Interacting Domain (ACID) of Human Mediator Subuniti 25. Northeast Structural Genomics Consortium Target HR6188A


Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Mediator of RNA polymerase II transcription subunit 25 Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q71SY5 (Residues: 391-543; Coverage: 21%)
Gene names: ACID1, ARC92, MED25, PTOV2, TCBAP0758
Structure domains: Ku70; Chain: A; Domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 94%
Refinement method: simulated annealing
Chemical shifts: BMR17323  
Expression system: Escherichia coli BL21(DE3)