PDBe 2kxh

Solution NMR

Solution structure of the first two RRM domains of FIR in the complex with FBP Nbox peptide

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Poly(U)-binding-splicing factor PUF60 Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 21.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHX1 (Residues: 119-314; Coverage: 35%)
Gene names: FIR, PUF60, ROBPI, SIAHBP1
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits
Far upstream element-binding protein 1 Chain: B
Molecule details ›
Chain: B
Length: 31 amino acids
Theoretical weight: 3.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96AE4 (Residues: 27-52; Coverage: 4%)
Gene name: FUBP1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli