2kre

Solution NMR

Solution structure of E4B/UFD2A U-Box domain

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin conjugation factor E4 B Chain: A
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 11.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95155 (Residues: 1208-1302; Coverage: 7%)
Gene names: HDNB1, KIAA0684, UBE4B, UFD2
Sequence domains: U-box domain
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DISTANCE GEOMETRY, SIMULATED ANNEALING
Expression system: Escherichia coli