2kfn

X-ray diffraction
2.03Å resolution

KLENOW FRAGMENT WITH BRIDGING-SULFUR SUBSTRATE AND MANGANESE

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 605 amino acids
Theoretical weight: 68.19 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00582 (Residues: 324-928; Coverage: 65%)
Gene names: JW3835, b3863, polA, resA
Sequence domains:
Structure domains:
5'-D(*GP*CP*TP*TP*AP*(US1)P*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 7 nucleotides
Theoretical weight: 2.13 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P43
Unit cell:
a: 102.9Å b: 102.9Å c: 86.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.25
Expression systems:
  • Escherichia coli
  • Not provided