Solution NMR

Solution NMR structure of lipoprotein spr from Escherichia coli K12. Northeast Structural Genomics target ER541-37-162


Function and Biology Details

Reaction catalysed:
GlcNAc-MurNAc-L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine + H(2)O = GlcNAc-MurNAc-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + D-alanine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Murein DD-endopeptidase MepS/Murein LD-carboxypeptidase Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 15.58 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
  • Canonical: P0AFV4 (Residues: 63-188; Coverage: 78%)
Gene names: JW2163, b2175, mepS, spr, yeiV
Sequence domains: NlpC/P60 family
Structure domains: endopeptidase domain like (from Nostoc punctiforme)

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 89%
Refinement method: simulated annealing
Chemical shifts: BMR15603  
Expression system: Escherichia coli