2joa

Solution NMR

HtrA1 bound to an optimized peptide: NMR assignment of PDZ domain and ligand resonances

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine protease HTRA1 Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 11.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q92743 (Residues: 380-480; Coverage: 22%)
Gene names: HTRA, HTRA1, PRSS11
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain
Peptide H1-C1 Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 962 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 87%
Refinement method: torsion angle dynamics
Chemical shifts: BMR15154  
Expression systems:
  • Escherichia coli BL21
  • Not provided