› 2jkp

X-ray diffraction
1.99Å resolution

Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with castanospermine

Released:
DOI: 10.2210/pdb2jkp/pdb
PDB entry 2jkp coloured by chain, front view.
PDB entry 2jkp coloured by chain, side view.
PDB entry 2jkp coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Divergence of catalytic mechanism within a glycosidase family provides insight into evolution of carbohydrate metabolism by human gut flora.
Gloster TM, Turkenburg JP, Potts JR, Henrissat B, Davies GJ
Chem Biol 15 1058-67 (2008)
PMID: 18848471

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124615 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucan 1,4-alpha-glucosidase SusB Chains: A, B
Molecule details ›
Chains: A, B
Length: 727 amino acids
Theoretical weight: 83.31 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: G8JZS4 (Residues: 22-738; Coverage: 100%)
Gene names: BT_3703, susB
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CTS 2 x CTS
Ligand CA 2 x CA
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 74.566Å b: 111.988Å c: 104.134Å
α: 90° β: 100.89° γ: 90°
R-values:
R R work R free
0.176 0.173 0.227
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

The abundance and variety of carbohydrate-active enzymes in the human gut microbiota.
El Kaoutari et al. (2013)
13 more

1 mention without citation

Divergence of catalytic mechanism within a glycosidase family provides insight into evolution of carbohydrate metabolism by human gut flora.
Gloster et al. (2008)