› 2jjb

X-ray diffraction
1.9Å resolution

Family 37 trehalase from Escherichia coli in complex with casuarine-6- O-alpha-glucopyranose

Released:
DOI: 10.2210/pdb2jjb/pdb
PDB entry 2jjb coloured by chain, front view.
PDB entry 2jjb coloured by chain, side view.
PDB entry 2jjb coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Total syntheses of casuarine and its 6-O-alpha-glucoside: complementary inhibition towards glycoside hydrolases of the GH31 and GH37 families.
Cardona F, Parmeggiani C, Faggi E, Bonaccini C, Gratteri P, Sim L, Gloster TM, Roberts S, Davies GJ, Rose DR, Goti A
Chemistry 15 1627-36 (2009)
PMID: 19123216

Function and Biology Details

Reaction catalysed: 
Alpha,alpha-trehalose + H(2)O = beta-D-glucose + alpha-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic trehalase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 535 amino acids
Theoretical weight: 60.53 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P13482 (Residues: 31-565; Coverage: 100%)
Gene names: JW1186, b1197, osmA, treA
Sequence domains: Trehalase
Structure domains: Glycosyltransferase

Ligands and Environments

4 bound ligands:
Ligand 3CU 4 x 3CU
Ligand GLC 4 x GLC
Ligand SO4 38 x SO4
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 92.481Å b: 117.863Å c: 203.451Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.224
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Insect trehalase: physiological significance and potential applications.
Shukla et al. (2015)
3 more

2 mentions without citation

Crystal structure of β-L-arabinobiosidase belonging to glycoside hydrolase family 121.
Saito et al. (2020)
1 more