› 2ji5

X-ray diffraction
2.45Å resolution

Structure of UMP kinase from Pyrococcus furiosus complexed with UTP

Released:
DOI: 10.2210/pdb2ji5/pdb
PDB 2ji5 coloured by chain and viewed from the front.
PDB 2ji5 coloured by chain and viewed from the side.
PDB 2ji5 coloured by chain and viewed from the top.
Source organism: Pyrococcus furiosus
Entry authors: Marco-Marin C, Rubio V

Function and Biology Details

Reaction catalysed: 
ATP + UMP = ADP + UDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridylate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 24.76 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8U122 (Residues: 1-225; Coverage: 100%)
Gene names: PF1407, pyrH
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like

Ligands and Environments

1 bound ligand:
Ligand UTP 2 x UTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: I23
Unit cell:
a: 144.943Å b: 144.943Å c: 144.943Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links