› 2jhk

X-ray diffraction
1.75Å resolution

Structure of globular heads of M-ficolin complexed with N-acetyl-D- glucosamine

Released:
DOI: 10.2210/pdb2jhk/pdb
PDB 2jhk coloured by chain and viewed from the front.
PDB 2jhk coloured by chain and viewed from the side.
PDB 2jhk coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structural basis for innate immune sensing by M-ficolin and its control by a pH-dependent conformational switch.
Garlatti V, Martin L, Gout E, Reiser JB, Fujita T, Arlaud GJ, Thielens NM, Gaboriaud C
J. Biol. Chem. 282 35814-20 (2007)
PMID: 17897951

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ficolin-1 Chain: F
Molecule details ›
Chain: F
Length: 218 amino acids
Theoretical weight: 24.35 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O00602 (Residues: 109-326; Coverage: 73%)
Gene names: FCN1, FCNM
Sequence domains: Fibrinogen beta and gamma chains, C-terminal globular domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: R3
Unit cell:
a: 73.75Å b: 73.75Å c: 125.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.174 0.187
Expression system: Trichoplusia ni

Quick links

Citations

9 review citations

Ficolins and the Recognition of Pathogenic Microorganisms: An Overview of the Innate Immune Response and Contribution of Single Nucleotide Polymorphisms.
Bidula et al. (2019)
8 more

1 mention without citation

Histidine-mediated pH-sensitive regulation of M-ficolin:GlcNAc binding activity in innate immunity examined by molecular dynamics simulations.
Yang et al. (2011)