› 2jgr

X-ray diffraction
2.65Å resolution

Crystal structure of YegS in complex with ADP

Released:
DOI: 10.2210/pdb2jgr/pdb
PDB entry 2jgr coloured by chain, front view.
PDB entry 2jgr coloured by chain, side view.
PDB entry 2jgr coloured by chain, top view.
Source organism: Escherichia coli BL21
Primary publication:
Crystal structure of YegS, a homologue to the mammalian diacylglycerol kinases, reveals a novel regulatory metal binding site.
Bakali HM, Herman MD, Johnson KA, Kelly AA, Wieslander A, Hallberg BM, Nordlund P
J Biol Chem 282 19644-52 (2007)
PMID: 17351295

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipid kinase YegS Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 32.39 KDa
Source organism: Escherichia coli BL21
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P76407 (Residues: 1-299; Coverage: 100%)
Gene names: JW2070, b2086, yegS
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand POP 1 x POP
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P42212
Unit cell:
a: 124.968Å b: 124.968Å c: 54.163Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.249
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Regulation of sphingosine kinase and sphingolipid signaling.
Pitson SM. (2011)
3 more