› 2jg0

X-ray diffraction
1.5Å resolution

Family 37 trehalase from Escherichia coli in complex with 1- thiatrehazolin

Released:
DOI: 10.2210/pdb2jg0/pdb
PDB 2jg0 coloured by chain and viewed from the front.
PDB 2jg0 coloured by chain and viewed from the side.
PDB 2jg0 coloured by chain and viewed from the top.
Source organism: Escherichia coli K-12
Primary publication:
Molecular basis for trehalase inhibition revealed by the structure of trehalase in complex with potent inhibitors.
Gibson RP, Gloster TM, Roberts S, Warren RA, Storch de Gracia I, Garc��a A, Chiara JL, Davies GJ
Angew. Chem. Int. Ed. Engl. 46 4115-9 (2007)
PMID: 17455176

Function and Biology Details

Reaction catalysed: 
Alpha,alpha-trehalose + H(2)O = beta-D-glucose + alpha-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic trehalase Chain: A
Molecule details ›
Chain: A
Length: 535 amino acids
Theoretical weight: 60.53 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P13482 (Residues: 31-565; Coverage: 100%)
Gene names: JW1186, b1197, osmA, treA
Sequence domains: Trehalase
Structure domains: Glycosyltransferase

Ligands and Environments

1 bound ligand:
Ligand TTZ 1 x TTZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 49.184Å b: 101.719Å c: 102.509Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.147 0.189
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Bacterial α-diglucoside metabolism: perspectives and potential for biotechnology and biomedicine.
Garcia et al. (2021)
4 more

2 mentions without citation

Synthesis and biological evaluation of nojirimycin- and pyrrolidine-based trehalase inhibitors.
Bini et al. (2012)
1 more