X-ray diffraction
2.2Å resolution

C-terminal domain of influenza A virus polymerase PB2 subunit in complex with human importin alpha5


Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-5, N-terminally processed Chains: A, B
Molecule details ›
Chains: A, B
Length: 450 amino acids
Theoretical weight: 49.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P52294 (Residues: 66-512; Coverage: 83%)
Gene names: KPNA1, RCH2
Sequence domains: Armadillo/beta-catenin-like repeat
Structure domains: Leucine-rich Repeat Variant
Polymerase basic protein 2 Chains: D, E
Molecule details ›
Chains: D, E
Length: 83 amino acids
Theoretical weight: 8.99 KDa
Source organism: Influenza A virus
Expression system: Escherichia coli BL21
  • Canonical: P31345 (Residues: 678-759; Coverage: 11%)
Gene name: PB2
Structure domains: Defensin A-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 94.97Å b: 100.55Å c: 151.84Å
α: 90° β: 90° γ: 90°
R R work R free
0.208 0.206 0.247
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli BL21