X-ray diffraction
1.8Å resolution

Crystal structure of alpha-1,3 Galactosyltransferase (R365K) in complex with UDP and 2 manganese ion


Function and Biology Details

Reaction catalysed:
UDP-alpha-D-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
N-acetyllactosaminide alpha-1,3-galactosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 34.06 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P14769 (Residues: 80-368; Coverage: 79%)
Gene name: GGTA1
Sequence domains: Glycosyltransferase family 6
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: C2
Unit cell:
a: 122.824Å b: 68.251Å c: 44.732Å
α: 90° β: 90.19° γ: 90°
R R work R free
0.182 0.182 0.192
Expression system: Escherichia coli BL21(DE3)