X-ray diffraction
1.53Å resolution

Deacetoxycephalosporin C synthase complexed with 5-hydroxy-4-keto valeric acid


Function and Biology Details

Reaction catalysed:
Penicillin N + 2-oxoglutarate + O(2) = deacetoxycephalosporin C + succinate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Deacetoxycephalosporin C synthase Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 34.59 KDa
Source organism: Streptomyces clavuligerus
Expression system: Escherichia coli
  • Canonical: P18548 (Residues: 1-311; Coverage: 100%)
Gene name: cefE
Sequence domains:
Structure domains: B-lactam Antibiotic, Isopenicillin N Synthase; Chain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: R3
Unit cell:
a: 106.5Å b: 106.5Å c: 71.2Å
α: 90° β: 90° γ: 120°
R R work R free
0.212 0.21 0.249
Expression system: Escherichia coli