› 2jas

X-ray diffraction
2.7Å resolution

Structure of deoxyadenosine kinase from M.mycoides with bound dATP

Released:
DOI: 10.2210/pdb2jas/pdb
PDB entry 2jas coloured by chain, front view.
PDB entry 2jas coloured by chain, side view.
PDB entry 2jas coloured by chain, top view.
Source organism: Mycoplasma mycoides subsp. mycoides SC
Primary publication:
Structure-function analysis of a bacterial deoxyadenosine kinase reveals the basis for substrate specificity.
Welin M, Wang L, Eriksson S, Eklund H
J Mol Biol 366 1615-23 (2007)
PMID: 17229440

Function and Biology Details

Reaction catalysed: 
ATP + deoxyguanosine = ADP + dGMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188074 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxynucleoside kinase domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 206 amino acids
Theoretical weight: 24.43 KDa
Source organism: Mycoplasma mycoides subsp. mycoides SC
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93IG4 (Residues: 1-205; Coverage: 100%)
Gene name: dGUOK
Sequence domains: Deoxynucleoside kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand DTP 6 x DTP
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 190.845Å b: 100.85Å c: 109.486Å
α: 90° β: 124.58° γ: 90°
R-values:
R R work R free
0.235 0.233 0.274
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

The Mysterious Multitude: Structural Perspective on the Accessory Subunits of Respiratory Complex I.
Padavannil et al. (2021)
4 other articles