2jai

X-ray diffraction
2.3Å resolution

DDAH1 complexed with citrulline

Released:

Function and Biology Details

Reaction catalysed:
N(omega),N(omega)-dimethyl-L-arginine + H(2)O = dimethylamine + L-citrulline
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 31.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O94760 (Residues: 1-285; Coverage: 100%)
Gene names: DDAH, DDAH1
Sequence domains: Amidinotransferase
Structure domains: L-arginine/glycine Amidinotransferase; Chain A

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 44.831Å b: 46.799Å c: 147.787Å
α: 90° β: 94.64° γ: 90°
R-values:
R R work R free
0.201 0.198 0.258
Expression system: Escherichia coli