› 2j4w

X-ray diffraction
2.5Å resolution

Structure of a Plasmodium vivax apical membrane antigen 1-Fab F8.12.19 complex

Released:
DOI: 10.2210/pdb2j4w/pdb
PDB 2j4w coloured by chain and viewed from the front.
PDB 2j4w coloured by chain and viewed from the side.
PDB 2j4w coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Cross-reactivity studies of an anti-Plasmodium vivax apical membrane antigen 1 monoclonal antibody: binding and structural characterisation.
Igonet S, Vulliez-Le Normand B, Faure G, Riottot MM, Kocken CH, Thomas AW, Bentley GA
J. Mol. Biol. 366 1523-37 (2007)
PMID: 17229439

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Apical membrane antigen 1 Chain: D
Molecule details ›
Chain: D
Length: 445 amino acids
Theoretical weight: 51.36 KDa
Source organism: Plasmodium vivax
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9TY14 (Residues: 1-445; Coverage: 100%)
Gene name: AMA-1
Sequence domains: Apical membrane antigen 1
Ig-like domain-containing protein Chain: H
Molecule details ›
Chain: H
Length: 225 amino acids
Theoretical weight: 24.19 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q569B4 (Residues: 20-123, 124-244; Coverage: 50%)
Gene name: Ighg
Sequence domains:
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.29 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q5XFY8 (Residues: 23-235; Coverage: 100%)
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P63
Unit cell:
a: 171.787Å b: 171.787Å c: 44.748Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.187 0.241
Expression system: Komagataella pastoris

Quick links

Citations

8 review citations

The Case for Exploiting Cross-Species Epitopes in Malaria Vaccine Design.
Mitran et al. (2020)
7 more

9 mentions without citation

The apicomplexan glideosome and adhesins - Structures and function.
Boucher et al. (2015)
8 more