› 2j3i

X-ray diffraction
2.8Å resolution

Crystal structure of Arabidopsis thaliana Double Bond Reductase (AT5G16970)-Binary Complex

Released:
DOI: 10.2210/pdb2j3i/pdb
PDB entry 2j3i coloured by chain, front view.
PDB entry 2j3i coloured by chain, side view.
PDB entry 2j3i coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Mechanistic and structural studies of apoform, binary, and ternary complexes of the Arabidopsis alkenal double bond reductase At5g16970.
Youn B, Kim SJ, Moinuddin SG, Lee C, Bedgar DL, Harper AR, Davin LB, Lewis NG, Kang C
J Biol Chem 281 40076-88 (2006)
PMID: 17028190

Function and Biology Details

Reaction catalysed: 
A n-alkanal + NAD(P)(+) = an alk-2-enal + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174436 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH-dependent oxidoreductase 2-alkenal reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 38.18 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q39172 (Residues: 1-345; Coverage: 100%)
Gene names: AER, At5g16970, F2K13_120, P1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P212121
Unit cell:
a: 49.15Å b: 122.66Å c: 147.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 not available
Expression system: Escherichia coli

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Citations

4 review citations

Dissection of lignin macromolecular configuration and assembly: comparison to related biochemical processes in allyl/propenyl phenol and lignan biosynthesis.
Davin et al. (2008)
3 more