› 2j3e

X-ray diffraction
3.2Å resolution

Dimerization is important for the GTPase activity of chloroplast translocon components atToc33 and psToc159

Released:
DOI: 10.2210/pdb2j3e/pdb
PDB 2j3e coloured by chain and viewed from the front.
PDB 2j3e coloured by chain and viewed from the side.
PDB 2j3e coloured by chain and viewed from the top.
Source organism: Arabidopsis thaliana
Primary publication:
Dimerization is important for the GTPase activity of chloroplast translocon components atToc33 and psToc159.
Yeh YH, Kesavulu MM, Li HM, Wu SZ, Sun YJ, Konozy EH, Hsiao CD
J. Biol. Chem. 282 13845-53 (2007)
PMID: 17337454

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Translocase of chloroplast 33, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 27.6 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O23680 (Residues: 2-250; Coverage: 84%)
Gene names: At1g02280, PPI1, T7I23.11, TOC33
Sequence domains: AIG1 family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL17B2
Spacegroup: P43212
Unit cell:
a: 71.521Å b: 71.521Å c: 106.269Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.291
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

The TOC GTPase Receptors: Regulators of the Fidelity, Specificity and Substrate Profiles of the General Protein Import Machinery of Chloroplasts.
Schnell DJ. (2019)
9 more

2 mentions without citation

One fold, two functions: cytochrome P460 and cytochrome c'-β from the methanotroph Methylococcus capsulatus (Bath).
Adams et al. (2019)
1 more