2j0x

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF E. COLI ASPARTOKINASE III IN COMPLEX WITH LYSINE AND ASPARTATE (T-STATE)

Released:
Source organism: Escherichia coli
Entry authors: Kotaka M, Ren J, Lockyer M, Hawkins AR, Stammers DK

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysine-sensitive aspartokinase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 449 amino acids
Theoretical weight: 48.58 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08660 (Residues: 1-449; Coverage: 100%)
Gene names: JW3984, apk, b4024, lysC
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 171.75Å b: 148.02Å c: 42.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.283
Expression system: Escherichia coli BL21(DE3)