2j0u

X-ray diffraction
3Å resolution

The crystal structure of eIF4AIII-Barentsz complex at 3.0 A resolution

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Eukaryotic initiation factor 4A-III Chain: A
Molecule details ›
Chain: A
Length: 374 amino acids
Theoretical weight: 42.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38919 (Residues: 38-411; Coverage: 91%)
Gene names: DDX48, EIF4A3, KIAA0111
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Eukaryotic initiation factor 4A-III Chain: B
Molecule details ›
Chain: B
Length: 374 amino acids
Theoretical weight: 42.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38919 (Residues: 38-411; Coverage: 91%)
Gene names: DDX48, EIF4A3, KIAA0111
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Protein CASC3 Chain: T
Molecule details ›
Chain: T
Length: 114 amino acids
Theoretical weight: 13.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15234 (Residues: 137-250; Coverage: 16%)
Gene names: CASC3, MLN51
Sequence domains: CASC3/Barentsz eIF4AIII binding

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 71.76Å b: 107.19Å c: 243.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.276 0.273 0.328
Expression system: Escherichia coli BL21(DE3)