› 2iyf

X-ray diffraction
1.7Å resolution

The crystal structure of macrolide glycosyltransferases: A blueprint for antibiotic engineering

Released:
DOI: 10.2210/pdb2iyf/pdb
PDB 2iyf coloured by chain and viewed from the front.
PDB 2iyf coloured by chain and viewed from the side.
PDB 2iyf coloured by chain and viewed from the top.
Source organism: Streptomyces antibioticus
Primary publication:
The crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity.
Bolam DN, Roberts S, Proctor MR, Turkenburg JP, Dodson EJ, Martinez-Fleites C, Yang M, Davis BG, Davies GJ, Gilbert HJ
Proc. Natl. Acad. Sci. U.S.A. 104 5336-41 (2007)
PMID: 17376874

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oleandomycin glycosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 415 amino acids
Theoretical weight: 45.39 KDa
Source organism: Streptomyces antibioticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53685 (Residues: 1-415; Coverage: 97%)
Gene names: UGT102A2, oleD
Sequence domains: UDP-glucoronosyl and UDP-glucosyl transferase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

3 bound ligands:
Ligand ERY 2 x ERY
Ligand UDP 2 x UDP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P21
Unit cell:
a: 72.718Å b: 65.778Å c: 91.94Å
α: 90° β: 100.42° γ: 90°
R-values:
R R work R free
0.201 0.2 0.232
Expression system: Escherichia coli BL21(DE3)

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Citations

16 review citations

GDP-Mannose 3,5-Epimerase: A View on Structure, Mechanism, and Industrial Potential.
Beerens et al. (2021)
15 more

15 mentions without citation

Understanding substrate selectivity of human UDP-glucuronosyltransferases through QSAR modeling and analysis of homologous enzymes.
Dong et al. (2012)
14 more