› 2iy3

Electron Microscopy
16Å resolution

Structure of the E. Coli Signal Regognition Particle

Released:
DOI: 10.2210/pdb2iy3/pdb
PDB 2iy3 coloured by chain and viewed from the front.
PDB 2iy3 coloured by chain and viewed from the side.
PDB 2iy3 coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Structure of the E. coli signal recognition particle bound to a translating ribosome.
Schaffitzel C, Oswald M, Berger I, Ishikawa T, Abrahams JP, Koerten HK, Koning RI, Ban N
Nature 444 503-6 (2006)
PMID: 17086205
Related structures: EMD-1250

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Signal recognition particle 54 kDa protein; Signal recognition particle protein Chain: A
Molecule details ›
Chain: A
Length: 432 amino acids
Theoretical weight: 48.27 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97ZE7 (Residues: 297-432; Coverage: 30%)
  • Canonical: O07347 (Residues: 1-296; Coverage: 69%)
Gene names: SSO0971, ffh, srp54
Sequence domains:
SIGNAL SEQUENCE Chain: C
Molecule details ›
Chain: C
Length: 17 amino acids
Theoretical weight: 1.38 KDa
Source organism: synthetic construct
Expression system: Not provided
4.5S RNA Chain: B
Molecule details ›
Chain: B
Length: 110 nucleotides
Theoretical weight: 35.55 KDa
Sequence domains: Bacterial small signal recognition particle RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 16Å
Relevant EMDB volumes: EMD-1250
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

21 review citations

Dynamics of co-translational protein targeting.
Elvekrog et al. (2015)
20 more

2 mentions without citation

Conformation of the signal recognition particle in ribosomal targeting complexes.
Buskiewicz et al. (2009)
1 more