2iy3

Electron Microscopy
16Å resolution

Structure of the E. Coli Signal Regognition Particle

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Signal recognition particle 54 kDa protein; Signal recognition particle protein Chain: A
Molecule details ›
Chain: A
Length: 432 amino acids
Theoretical weight: 48.27 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97ZE7 (Residues: 297-432; Coverage: 30%)
  • Canonical: O07347 (Residues: 1-296; Coverage: 69%)
Gene names: SSO0971, ffh, srp54
Sequence domains:
SIGNAL SEQUENCE Chain: C
Molecule details ›
Chain: C
Length: 17 amino acids
Theoretical weight: 1.38 KDa
Source organism: synthetic construct
Expression system: Not provided
4.5S RNA Chain: B
Molecule details ›
Chain: B
Length: 110 nucleotides
Theoretical weight: 35.55 KDa
Sequence domains: Bacterial small signal recognition particle RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 16Å
Relevant EMDB volumes: EMD-1250
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided