X-ray diffraction
1.5Å resolution

Crystal structure of human ABH3 in complex with iron ion and 2- oxoglutarate


Function and Biology Details

Reaction catalysed:
N(1)-methyladenine in mRNA + 2-oxoglutarate + O(2) = adenine in mRNA + formaldehyde + succinate + CO(2)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 27.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q96Q83 (Residues: 62-286; Coverage: 79%)
Gene names: ABH3, ALKBH3, DEPC1
Sequence domains: 2OG-Fe(II) oxygenase superfamily
Structure domains: Alpha-ketoglutarate-dependent dioxygenase AlkB-like

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I41
Unit cell:
a: 119.955Å b: 119.955Å c: 40.554Å
α: 90° β: 90° γ: 90°
R R work R free
0.14 not available 0.191
Expression system: Escherichia coli BL21(DE3)