› 2iu4

X-ray diffraction
1.96Å resolution

Dihydroxyacetone kinase operon co-activator Dha-DhaQ

Released:
DOI: 10.2210/pdb2iu4/pdb
PDB entry 2iu4 coloured by chain, front view.
PDB entry 2iu4 coloured by chain, side view.
PDB entry 2iu4 coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators.
Christen S, Srinivas A, Bähler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B
J Biol Chem 281 23129-37 (2006)
PMID: 16760471

Function and Biology Details

Reaction catalysed: 
ATP + D-glucose = ADP + D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189979 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DhaKLM operon coactivator DhaQ Chains: A, B
Molecule details ›
Chains: A, B
Length: 336 amino acids
Theoretical weight: 37.44 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9CIW0 (Residues: 1-328; Coverage: 100%)
Gene names: L44063, LL0246, dhaQ
Sequence domains: Dak1 domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand HIQ 2 x HIQ

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 99.897Å b: 99.897Å c: 188.566Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.181 0.209
Expression system: Escherichia coli

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Citations

1 review citation

The TetR family of regulators.
Cuthbertson et al. (2013)
27 other articles