PDBe 2iu0

X-ray diffraction
2.53Å resolution

crystal structures of transition state analogue inhibitors of inosine monophosphate cyclohydrolase


Function and Biology Details

Reactions catalysed:
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
IMP + H(2)O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Bifunctional purine biosynthesis protein PURH Chains: A, B
Molecule details ›
Chains: A, B
Length: 593 amino acids
Theoretical weight: 64.5 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P31335 (Residues: 1-593; Coverage: 100%)
Gene names: ATIC, PURH
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2
Unit cell:
a: 387Å b: 57Å c: 62.1Å
α: 90° β: 98.9° γ: 90°
R R work R free
0.209 0.209 0.261
Expression system: Escherichia coli BL21(DE3)