X-ray diffraction
1.8Å resolution

Crystal structure of h3alpha-hydroxysteroid dehydrogenase type 3 mutant Y24A in complex with NADP+ and epi-testosterone


Function and Biology Details

Reactions catalysed:
Trans-1,2-dihydrobenzene-1,2-diol + NADP(+) = catechol + NADPH
17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H
Indan-1-ol + NAD(P)(+) = indanone + NAD(P)H
A 3-alpha-hydroxysteroid + NAD(P)(+) = a 3-oxosteroid + NAD(P)H
Androstan-3-alpha,17-beta-diol + NAD(+) = 17-beta-hydroxyandrostan-3-one + NADH
Androsterone + NAD(P)(+) = 5-alpha-androstane-3,17-dione + NAD(P)H
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Aldo-keto reductase family 1 member C2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 321 amino acids
Theoretical weight: 36.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P52895 (Residues: 3-323; Coverage: 99%)
Gene names: AKR1C2, DDH2
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments

Cofactor: Ligand NAP 2 x NAP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A, null
Spacegroup: R32
Unit cell:
a: 144.788Å b: 144.788Å c: 203.885Å
α: 90° β: 90° γ: 120°
R R work R free
0.204 0.204 0.228
Expression system: Escherichia coli