X-ray diffraction
2.7Å resolution

Crystal structure of lactaldehyde dehydrogenase from E. coli: the binary complex with NADPH


Function and Biology Details

Reactions catalysed:
(S)-lactaldehyde + NAD(+) + H(2)O = (S)-lactate + NADH
Glycolaldehyde + NAD(+) + H(2)O = glycolate + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Lactaldehyde dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 479 amino acids
Theoretical weight: 52.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
  • Canonical: P25553 (Residues: 1-479; Coverage: 100%)
Gene names: JW1412, ald, aldA, b1415
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

Cofactor: Ligand NDP 1 x NDP
1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P6422
Unit cell:
a: 143.566Å b: 143.566Å c: 108.763Å
α: 90° β: 90° γ: 120°
R R work R free
0.212 0.212 0.253
Expression system: Escherichia coli