› 2ilu

X-ray diffraction
2.7Å resolution

Crystal structure of lactaldehyde dehydrogenase from E. coli: the binary complex with NADPH

Released:
DOI: 10.2210/pdb2ilu/pdb
PDB 2ilu coloured by chain and viewed from the front.
PDB 2ilu coloured by chain and viewed from the side.
PDB 2ilu coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Crystal structure of lactaldehyde dehydrogenase from Escherichia coli and inferences regarding substrate and cofactor specificity.
Di Costanzo L, Gomez GA, Christianson DW
J. Mol. Biol. 366 481-93 (2007)
PMID: 17173928

Function and Biology Details

Reactions catalysed: 
(S)-lactaldehyde + NAD(+) + H(2)O = (S)-lactate + NADH
Glycolaldehyde + NAD(+) + H(2)O = glycolate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactaldehyde dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 479 amino acids
Theoretical weight: 52.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P25553 (Residues: 1-479; Coverage: 100%)
Gene names: JW1412, ald, aldA, b1415
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand OCS 1 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P6422
Unit cell:
a: 143.566Å b: 143.566Å c: 108.763Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.212 0.253
Expression system: Escherichia coli

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Citations

3 review citations

Biotechnological routes based on lactic acid production from biomass.
Gao et al. (2011)
2 more

3 mentions without citation

Crystal structure of lactaldehyde dehydrogenase from Escherichia coli and inferences regarding substrate and cofactor specificity.
Di Costanzo et al. (2007)
2 more