2iip

X-ray diffraction
2.05Å resolution

Human Nicotinamide N-methyltransferase

Released:
Source organism: Homo sapiens
Entry authors: Bernstein G, Min J, Wu H, Tempel W, Zeng H, Loppnau P, Avvakumov GV, Wasney G, Weigelt J, Sundstrom M, Arrowsmith CH, Edwards AM, Bochkarev A, Plotnikov AN, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + nicotinamide = S-adenosyl-L-homocysteine + 1-methylnicotinamide
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamide N-methyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 283 amino acids
Theoretical weight: 31.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P40261 (Residues: 1-264; Coverage: 100%)
Gene name: NNMT
Sequence domains: NNMT/PNMT/TEMT family
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 4 x SAH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P1
Unit cell:
a: 46.016Å b: 62.007Å c: 107.073Å
α: 82.35° β: 81.89° γ: 68.3°
R-values:
R R work R free
0.219 0.217 0.265
Expression system: Escherichia coli