2iie

X-ray diffraction
2.41Å resolution

single chain Integration Host Factor protein (scIHF2) in complex with DNA

Released:
Source organism: Escherichia coli K-12
Primary publication:
A divalent metal-mediated switch controlling protein-induced DNA bending.
J. Mol. Biol. 367 731-40 (2007)
PMID: 17276457

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Integration host factor subunit alpha; Integration host factor subunit beta Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 22.88 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6X7 (Residues: 3-94; Coverage: 93%)
  • Canonical: P0A6Y1 (Residues: 2-39, 40-94; Coverage: 99%)
Gene names: JW0895, JW1702, b0912, b1712, hid, himA, himD, hip, ihfA, ihfB
Sequence domains: Bacterial DNA-binding protein
Structure domains: IHF-like DNA-binding proteins
Phage P H' site Chain: C
Molecule details ›
Chain: C
Length: 35 nucleotides
Theoretical weight: 10.8 KDa
DNA (5'-D(*DGP*DGP*DCP*DCP*DAP*DAP*DAP*DAP*DAP*DAP*DGP*DCP*DAP*DTP*DT)-3') Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.61 KDa
DNA (5'-D(*DGP*DCP*DTP*DTP*DAP*DTP*DCP*DAP*DAP*DTP*DTP*DTP*DGP*DTP*DTP*DGP*DCP*DAP*DCP*DC)-3') Chain: E
Molecule details ›
Chain: E
Length: 20 nucleotides
Theoretical weight: 6.07 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 47.371Å b: 55.311Å c: 177.833Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.233 0.272
Expression system: Escherichia coli