2ihm

X-ray diffraction
2.4Å resolution

Polymerase mu in ternary complex with gapped 11mer DNA duplex and bound incoming nucleotide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA-directed DNA/RNA polymerase mu Chains: A, B
Molecule details ›
Chains: A, B
Length: 360 amino acids
Theoretical weight: 40.61 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9JIW4 (Residues: 137-496; Coverage: 73%)
Gene names: Polm, polmu
Sequence domains:
Structure domains:
5'-D(*CP*GP*GP*CP*AP*AP*TP*AP*CP*TP*G)-3' Chains: T, U
Molecule details ›
Chains: T, U
Length: 11 nucleotides
Theoretical weight: 3.36 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(*CP*AP*GP*TP*AP*T)-3' Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 6 nucleotides
Theoretical weight: 1.81 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(P*GP*CP*CP*G)-3' Chains: D, E
Molecule details ›
Chains: D, E
Length: 4 nucleotides
Theoretical weight: 1.19 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 71.028Å b: 96.085Å c: 73.09Å
α: 90° β: 106.55° γ: 90°
R-values:
R R work R free
0.232 0.23 0.277
Expression systems:
  • Escherichia coli
  • Not provided